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Data AnalysisThe flowchart that outlines the analysis of 16S rRNA sequence reads is shown in Fig. 1a. Then barcode sequences of forward read (BC F) and reverse read (BC R) were joined and brought together (5 end) with the assembled sequence (3 end) after discarding 8 nucleotide random sequence and 27F and 533R primer sequences using custom Perl script, which produced fastq file compatible to downstream analysis using QIIME..
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While driver diagrams have yet to be used specifically for evaluation, they have been widely used to guide implementation in a systematic way. An example is the 20 000+ Partnership, a regional initiative in KwaZulu Natal, South Africa, that aims to reduce mother to child HIV transmission rates to less than 5%. This project’s initial driver diagram outlined the spectrum of activities that the implementers intended to introduce.